Device

Part:BBa_K4728022:Design

Designed by: Bryant Han   Group: iGEM23_Queens-Canada   (2023-10-10)


TPA detection device (with the transporter TpaK)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2829
    Illegal PstI site found at 80
    Illegal PstI site found at 2935
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2829
    Illegal NheI site found at 958
    Illegal NheI site found at 981
    Illegal PstI site found at 80
    Illegal PstI site found at 2935
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2829
    Illegal BglII site found at 1591
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2829
    Illegal PstI site found at 80
    Illegal PstI site found at 2935
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2829
    Illegal PstI site found at 80
    Illegal PstI site found at 2935
    Illegal AgeI site found at 1363
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Depending on how one assembles the transcriptional units into this level 2 part, the scars may differ.


Source

TpaK comes from Rhodococcus jostii's genome and TphR from Comamonas testosteroni.

References